Determining the timing and location of breeding events is important for understanding species’ biology for effective wildlife management. Many aquatic organisms breed through spawning, with males releasing a vast quantity of spermatozoa into the waterway. eDNA surveys were able to successfully detect a change in the relative abundance of nuclear and mitochondrial DNA in laboratory and field studies, enabling identification of spawning activity in the endangered Macquarie perch, Macquaria australasica. This demonstrates the potential of the eDNA method for detecting reproductive activity in aquatic organisms to increase knowledge of the reproductive biology of elusive species.
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